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A simple, sensitive, and accurate
spectrophotometric method has been developed for the determination
of riboflavin in pure and pharmaceutical formulations. The method is
based on the reaction of riboflavin with sodium nitroprusside in the
presence of hydroxylamine hydrochloride and sodium carbonate to give
an intense greenish yellow colored species which absorb maximally at
445 nm. Beer’s law is obeyed in the concentration range of 0.94–7.52
ppm with a coefficient of determination r2=0.9996.
Statistical analysis of this method exhibited relative standard
deviation of 1.28% with the limit of detection 0.564 ppm and limit
of determination 1.690 ppm. The proposed method has been applied
successfully for the determination of riboflavin in pharmaceutical
products and can be extended for the routine analysis in bulk drugs. |
