Indian Journal of Advances in Chemical Science, Volume: 8, Issue :4 , December 2020

ISSN No.: 2320-0898 (Print); 2320-0928 (Electronic)

DOI: 10.22607/IJACS.2020.804004

Research Article

Anthocyanins Characterization and Antioxidant Activities of Odontonema
strictum (Nees) Kuntze Flowers

ABSTRACT

In the last decades, the interest in anthocyanin pigments has enhanced due to their possible use as natural food colorants and mostly as antioxidant agents. Anthocyanins in flowers of Odontonema strictum (Nees) Kuntze were characterized by highperformance liquid chromatography combined with electrospray ionization quadrupole-time-of-flight mass spectrometry. A total of five anthocyanins were identified. They were reported from O. strictum flowers for the 1st time. O. strictum flowers extracts were also investigated for their total phenolic, flavonoid, anthocyanin contents, and antioxidant activities using the 2,2-diphenyl1-picrylhydrazyl radical scavenging capacity assay. Using the Folin-Ciocalteu’s reagent, the highest total phenolic content from flowers was obtained with methanol extract (84.0 ± 3.0 mg AGE/g dry weight). The total anthocyanin content (TAC) showed a similar trend (0.3 ± 0.03 mg cyanidin-3-glucoside/g dry weight) for methanol extract. In line with the total phenolic and TAC, the methanol extract of O. strictum flowers presents the highest antioxidant capacity (IC50 = 0.28 ± 0.03 mg/mL). The highest total flavonoid content was observed with ethyl acetate extract (29.0 ± 0.6 mg rutin equivalent/g dry weight). This study provides useful information for the production of valuable nutraceuticals from flowers of O. strictum (Nees) Kuntze.

Key words:  Odontonema strictum, Anthocyanins, High-performance liquid chromatography-Electrospray ionization-mass spectrometry, Antioxidants, Functional food.

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