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Dual responsive copolymeric poly(N-(2-aminothiazolyl)
maleamic acid-co-N-isopropyl acrylamide) nanogels (PAMNI-NGs)
produced from N-(2-aminothiazolyl) maleamic acid (AMA) and
N-isopropyl acrylamide (NIPAM) through a simple free radical
emulsion polymerization. The formations of PAMNI-NGs were confirmed
by Fourier-transform-infrared (FTIR) spectroscopy, dynamic light
scattering (DLS), transmission electron microscopy (TEM), and atomic
force microscopy (AFM). TEM and DLS results gave the surface
morphology and particle size, respectively, that PAMNI-NGs are semi
slightly spherical and are found around 100 nm, further it is
confirmed with AFM. Further, Zidovudine (ZDV), an anti-human
immunodeficiency virus drug, was successfully loaded into the
developed PAMNI-NGs using an equilibrium swelling method. The
formation of PAMNI-NGs was investigated through FTIR, DLS, AFM, and
transmission electron microscopy (TEM) analyses, while the ZDV-loaded
PAMNINGs were confirmed from FTIR and XRD studies. The encapsulation
efficiency of ZDV is varied from 33 to 82%. The in vitro release
studies of ZDV from the PAMNI-NGs were examined in different
gastrointestinal tract pH conditions (pH 1.2 and 7.4) at 25° and
37°C. The presence of aminothiazole functionality in the PAMNI-NGs
also showed excellent antibacterial activity towards bacterial
strains of Gram-positive (Bacillus cereus and Staphylococcus aureus)
and Gram-negative (Escherichia coli and Klebsiella
pneumoniae).Therefore, the in vitro release and antibacterial
studies revealed the efficiency of PAMNI-NGs toward site specific
drug delivery and antimicrobial applications, respectively. |
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Key words: N-(2-aminothiazolyl) maleamic acid, N-isopropyl
acrylamide, Copolymer, Nanogels, Controlled release,
Antibacterial activity. |
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