Indian Journal of Advances in Chemical Science, Volume: 11, Issue : 1, January 2023

ISSN No.: 2320-0898 (Print); 2320-0928 (Electronic)

DOI:10.22607/IJACS.2023.1101007

Research Article

Isolation of Carbosulfan Degrading Lysinibacillus fusiformis from Paddy (Oryza sativa L.) Soil

K. Swetha, V. Satyanrayana Swamy, M. Srinivasulu, P. Ramanamma, U. Keerthi, D. Lumbini Devi, D. Muralidhara Rao*
 

ABSTRACT

A branded generic insecticide with a long history of usage in agriculture is carbosulfan. However, research indicated that carbosulfan may be very hazardous to aquatic life. Using the enrichment culture approach, a bacterial culture was isolated from soil samples that had received the insecticide carbosulfan 5 times at a concentration of 5 kg/ha. This bacterial culture was then examined to see how well the pesticide broke down in a mineral salts medium. Based on the 16S rRNA gene sequence, the isolate was recognized as a Lysinibacillus fusiformis. In terms of phylogenetic analysis and nucleotide homology, the bacteria were quite similar. In conditions with increased amounts of carbosulfan, the bacteria acquired the ability to thrive. The creation of multiresistant microorganisms that may be used to return the altered environment to its previous condition may, therefore, benefit from these findings. To eliminate the carbosulfan residues from the medium, an equivalent quantity of dichloromethane was utilized. After the required intervals, the culture filtrate was redissolved in about 10 mL of acetonitrile for the high performance liquid chromatography analysis in the present investigation (0, 1st, 2nd, 3rd, and 4th day).
 

Key words: Carbosulfan, Paddy soil, Lysinibacillus fusiformis, High-performance liquid chromatography.
 

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