Cellulase Production Using Biomass Feed Stocks by Indigenous Strains

DOI: 10.22607/IJACS.2024.1202009

Research  Article

ABSTRACT

The feasible construction of chemicals and value-added products from inexhaustible, non-edible biomass has become a critical alternative to undertake pressing environmental problems created by our overdependence on fossil resources. The special strains of microorganisms called microbial cell factories are able to produce chemicals of interested through renewable carbon sources. Lignocellulosic biomass is considered a significant of such materials which can be used for the production of useful materials that are not only renewable but also do not have any edible values. This work reveals the ability of bacteria and fungi isolated from mango field soil samples to produce cellulolytic enzymes. In total, more than 100 bacterial colonies were obtained and 13 fungal strains were identified from the mango field soil sample. From these organisms, we choose one bacterium strain and one fungus strain after the secondary screening for hypercellulolytic microorganisms such as Proteus mirabilis and Aspergillus. Black gram husk, red cowpea husk, Bengal gram husk, and groundnut fodder were tested for cellulase production by bacteria under submerged condition fermentation and in solid-state fermentation with fungal strains. Red cowpea husk and identification of these cultures were done using biochemical and morphological features, and ground nut husk is the recommended solid substrate for maximum production of β-glucosidase, protein content and filter paperase (FPase) and carboxymethyl cellulase,
respectively, by bacterial isolate.

Key words: Agricultural residues, Bacteria, Cellulase, Fungi, Solid-state fermentation, Submerged fermentation.

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Indian Journal of Advances in Chemical Science,

Volume: 12, Issue : 2,  April 2024

ISSN No.: 2320-0898 (Print); 2320-0928 (Electronic)