Indian Journal of Advances in Chemical Science Volume: 6, Issue 3: , August 2018

ISSN No.: 2320-0898 (Print); 2320-0928 (Electronic)

DOI: 10.22607/IJACS.2018.603010

Research Article

Synthesis of Silver Nanocomposite Hydrogel from Lysozyme-Mediated Poly(acrylamide) for the Inactivation of Pathogenic Bacteria

G. Viswanatha Reddy, K. Madhusudana Rao, K. S. V. Krishna Rao*, Chang-Sik Ha*

ABSTRACT

A simple eco-friendly method involving the lysozyme (Lys)-mediated controllable synthesis of silver nanoparticles (AgNPs) in poly(acrylamide) (PAAm) hydrogel networks is reported. The as-synthesized AgNPs present in the hydrogels were characterized by Fourier-transform infrared spectroscopy, UV-visible spectroscopy, X-ray diffraction, and scanning electron microscopy. Transmission electron microscopy and dynamic light scattering revealed the AgNPs to have a mean size of approximately 5–8 nm. The Lys-encapsulated hydrogel silver nanocomposite exhibited excellent antibacterial activity toward both Gram-positive and Gram-negative bacteria, which was superior to that of hydrogel silver nanocomposites produced by sodium borohydride reduction. The results highlight the potential of the Lys-mediated production of silver nanocomposites for antibacterial wound dressing applications.

Key words: Lysozyme, Hydrogel, Silver nanoparticles, Antibacterial activity, Wound dressing.

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