Indian Journal of Advances in Chemical Science Volume: 7, Issue :3 , August 2019

ISSN No.: 2320-0898 (Print); 2320-0928 (Electronic)

DOI: 10.22607/IJACS.2018.703001

Research Article

Decolorization of Triphenylmethane Dye by White-rot Fungi

K. Y. Usha, Arpita Dhar, Ridham, Sharangouda J. Patil, K. Praveen Kumar*

ABSTRACT

Decolorization of triphenylmethane dye (crystal violet) by white-rot fungi – Stereum ostrea and Phanerochaete chrysosporium was investigated in liquid medium for a period of 10 days under shaking conditions. S. ostrea exhibited maximum decolorization crystal violet 90% in comparison with P. chrysosporium that it was 67% at 0.01% concentration on the 8th day of incubation. S. ostrea dominates P. chrysosporium on the production of biomass and protein content. The fungal cultures were tested for the production of ligninolytic enzymes – laccase (Lcc), lignin peroxidase (LiP), and manganese peroxidase (MnP). Fungal cultures grew well in dye-containing medium and exhibited decolorization of crystal violet confirming the presence of ligninolytic enzymes. S. ostrea displayed maximum Lcc (49 U/ml), LiP (0.34 U/ml), and MnP (48 U/ml) against P. chrysosporium.

Key words: Decolorization, Triphenylmethane dye, Laccase, Manganese peroxidase, Lignin peroxidase, Biomass, Protein content.

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