Indian Journal of Advances in Chemical Science Volume: 7, Issue :3 , August 2019

ISSN No.: 2320-0898 (Print); 2320-0928 (Electronic)

DOI: 10.22607/IJACS.2018.703002

Research Article

Higher Production of Cellulase and Amylase by Novel Aspergillus sp. in Solid-State Fermentation

M. Subhosh Chandra*, T. Lokesh, C. Anusha, P. Suresh Yadav

ABSTRACT

A potentially novel Aspergillus sp. as a producer of multienzymes is exploited in fungal fermentation on lignocellulosic substrates. High-level production of extracellular cellulase (exoglucanase, endoglucanase, and β-glucosidase) and amylase has been achieved. Three different types of “waste” solids such as sugarcane bagasse, sawdust, and tea residue have been used in solid-state fermentation (SSF). Among the three different lignocellulosic substrates used in this study, sugarcane bagasse was the excellent solid substrate for higher production of cellulase and amylase enzymes. Sugarcane bagasse supported remarkable production of filter paperase (15.42 FPU/g of substrate), carboxymethyl cellulase (CMCase) (17.89 U/g of substrate) and β-glucosidase (2.43 U/g of substrate), and amylase activity (15.12 U/g of substrate) in SSF. Significant secretion of protein (45.75 mg/g of substrate) on sugarcane bagasse was noticed. Though, producing sustainable biofuels from lignocellulosic substrates in a cost-effective manner, it is an important challenge in the commercialization of the production process.

Key words: Lignocellulosic substrates, Aspergillus sp., Exoglucanase, Endoglucanase, β-Glucosidase, Amylase

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