Indian Journal of Advances in Chemical Science Volume: 8, Issue :2 , April 2020

ISSN No.: 2320-0898 (Print); 2320-0928 (Electronic)

DOI: 10.22607/IJACS.2020.802006    

Research Article

Simultaneous Determination of Isoquercitrin and Astragalin in Plant (Leaf) Extract Using Liquid Chromatography with Tandem Mass Spectrometry Method for the Application of Toxicology Studies in Matrix

ABSTRACT

An analytical approach has been developed and validated using liquid chromatography (LC)-mass Spectrometry (MS)/MS for the simultaneous determination of isoquercitrin and astragalin from the TGT Primaage (plant [leaf] extract), following the application of toxicology studies. The purpose of analytical method validation was established a sensitive data, which was investigated for chronic toxicology studies evaluation. The method validations were implemented for determining the individuality and concentration of the analytes, matrix match effects and provide an estimated analytical method validation. The method validation was carried out by performing different parameters using reference standards and test substance solutions of isoquercitrin and astragalin in the matrix and analyzed onto LC-MS/MS. This analytical validated method was successfully applied to the actual samples of the toxicology studies for the dose formulation analysis of TGT Primaage (plant [leaf] extract) samples which had the contents of isoquercitrin and astragalin.

Key words: Flavonoid, Liquid chromatography-Mass spectrometry/mass spectrometry, Toxicology studies, International Conference on Harmonization Q2 (R1), Extraction technique, Matrix effect, Chemical compound.

[Download Full Article PDF]