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2-Acetylpyridine thiosemicarbazone
(APT) and 2-acetylpyridine semicarbazone (APS) have been used for
the spectrophotometric determination of zinc(II) in aqueous medium.
APT and APS react with zinc(II) in acidic medium. The color
reactions between reagents with zinc(II) are instantaneous and the
absorbance of complexes remains constant for over 24 h. The maximum
absorbance (lmax), composition (M: L), molar absorptivity, and
Sandells sensitivity of the Zn-APT and Zn-APS complexes,
respectively, are 360, 355 nm, 1:2, 4.06×104, 8.12×103 L/mol/cm, and
0.0160, 0.080 μgcm−2 of Zn(II), respectively. The Zn-APT and Zn-APS
systems obey Beer’s law for 0.105–1.046, and 0.523–5.231 μg/ml of
Zn(II), respectively. Large number of cations, anions, and
complexing agents (e.g. triethanolamine, and thiourea) does not
interfere in APT method. The method is successfully applied for the
determination of zinc in biological samples. |
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Key words: Key words:
Spectrophotometry, Zinc determination, 2-Acetylpyridine
thiosemicarbazone, 2-Acetylpyridine semicarbazone, Biological
samples. |
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