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Two visible spectrophotometric
methods were developed A and B for the determination of Tripolidine
Hydrochloride in pure and pharmaceutical formulations. Method A is
based on the formation of co-ordination complex of tertiary amine of
TPH (electron donor) and the central metal of the cobalt thiocyanate
(acceptor) and Method B is based on internal salt formation
involving aconitic anhydride (dehydration product of CiA) and the
tertiary amine of TPH. The coloured products exhibit absorption λmax
at 623 nm and 546nm. Regression analysis of Beer-Lambert plots
showed good correlation in the concentration ranges 8-48 μg/ml,
correlation co-efficients are 0.9999(A), 0.9822(B);the Sandell’s
sensitivities are 2.7705 x 10-3 , 1.5058 x 10-3
(1 mole cm-1); molar absorptivity values are1.1364 x 105
, 2.0908 x 105 (µg cm-2)for methods A,B
respectively.The proposed methods are applied to commercial
available formulations and the results are statistically compared
with those obtained by the UV reference method and validated by
recovery studies. The results are found satisfactory and
reproducible. These methods are applied successfully for the
estimation of the TPH in the presence of other ingredients that are
usually present in formulations. These methods offer the advantages
of rapidity, simplicity and sensitivity and low cost without the
need for expensive instrumentation
and reagents.
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